Supporting Information for Discrimination of Saturated Aldehydes by the Rat I7 Olfactory Receptor
نویسندگان
چکیده
Discrimination of Saturated Aldehydes by the Rat I7 Olfactory Receptor Michael D. Kurland1, Michael B. Newcomer1, Zita Peterlin3, Kevin Ryan2, Stuart Firestein3*, and Victor S. Batista1* Department of Chemistry, Yale University, New Haven, Connecticut 06520-8107; Department of Chemistry, City College of New York, New York, NY 10031, Department of Biological Sciences, Columbia University, New York, NY 10027
منابع مشابه
Discrimination of saturated aldehydes by the rat I7 olfactory receptor.
The discrimination of n-alkyl-saturated aldehydes during the early stage of odorant recognition by the rat I7 olfactory receptor (OR-I7) is investigated. The concentrations of odorants necessary for 50% activation (or inhibition) of the OR-I7 are measured by calcium imaging recordings of dissociated rat olfactory sensory neurons, expressing the recombinant OR-I7 from an adenoviral vector. These...
متن کاملCarbon chain shape selectivity by the mouse olfactory receptor OR-I7.
The rodent OR-I7 is an olfactory receptor exemplar activated by aliphatic aldehydes such as octanal. Normal alkanals shorter than heptanal bind OR-I7 without activating it and hence function as antagonists in vitro. We report a series of aldehydes designed to probe the structural requirements for aliphatic ligand chains too short to meet the minimum approximate 6.9 Å length requirement for rece...
متن کاملHaloferax volcanii, as a Novel Tool for Producing Mammalian Olfactory Receptors Embedded in Archaeal Lipid Bilayer
The aim of this study was to explore the possibility of using an archaeal microorganism as a host system for expressing mammalian olfactory receptors (ORs). We have selected the archaeon Haloferax volcanii as a cell host system and one of the most extensively investigated OR, namely I7-OR, whose preferred ligands are short-chain aldehydes, such as octanal, heptanal, nonanal. A novel plasmid has...
متن کاملIdentification of Ligands for Olfactory Receptors by Functional Expression of a Receptor Library
The recognition of odorants by olfactory receptors represents the first stage in odor discrimination. Here, we report the generation of an expression library containing a large and diverse repertoire of mouse olfactory receptor sequences in the transmembrane II-VII region. From this library, 80 chimeric receptors were tested against 26 odorants after transfection into HEK-293 cells. Three recep...
متن کاملRole of topology in electrical properties of bacterio-rhodopsin and rat olfactory receptor I7.
We report on electrical properties of the two sensing proteins: bacteriorhodopsin and rat olfactory receptor OR-I7. As relevant transport parameters we consider the small-signal impedance spectrum and the static current-voltage characteristics. Calculations are compared with available experimental results and the model predictability is tested for future perspectives.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2010